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New York State College of Agriculture and Life Sciences, Department of Animal Science, Cornell University, Ithaca,NY 14853
New York State College of Veterinary Medicine, Department of Clinical Studies, Cornell University, Ithaca, NY 14853
New York State College of Agriculture and Life Sciences, Department of Animal Science, Cornell University, Ithaca, NY 14853
ABSTRACT
A one-piece cannula was developed for reentry or spot collection of duodenal digesta in dairy cows. Progession through three cannula designs is described. Exteriorizing cannulae between the tenth and eleventh ribs produced stable preparations although ribs were deformed. A simple one-piece T cannula was suitable for spot sampling, but digesta blockage prevented its use for reentry collections. A double entry T cannula was unsuitable for reentry collections because of digesta blockages and inability to secure diversion gates in the cannula. A Y-shaped cannula allowed blockage-free reentry collections and easy spot sampling. Diversion gate design provided for a snug fit, and dualphase digesta marker recovery studies and necropsy findings suggested complete diversion in two of three cows. Post-mortem observations of rib distortion and intestinal tissue erosion from between the cannula and abdominal wall suggest inclusion of an inner restraining collar for future preparations. Several other modifications are discussed.
1 Institute for Livestock Feeding and Nutrition Research (IVVO), Runderweg 2, P.O. Box 160, 8200 AD Leiystad, Nederland.
2 Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison 53706.
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