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Journal of Dairy Science Vol. 68 No. 2 307-311
© 1985 by American Dairy Science Association ®
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Separation of Casein Micelles from Milk for Rapid Determination of Casein Concentration1

Robert N. Carpenter2 and Rodney J. Brown

Department of Nutrition and Food Sciences, Utah State University, Logan 84322

ABSTRACT

Size exclusion chromatography was used to separate casein micelles from whey protein in milk for rapid measurement of casein. Casein concentration was measured by size exclusion separation monitored by ultraviolet spectroscopy and by acid precipitation followed by Kjeldahl estimation of protein. The correlation coefficient between the two methods was .92. Effects of temperature, pH, and calcium on separation were evaluated. Addition of calcium was used to incorporate all casein into micelles before separation.

FOOTNOTES

1 Utah State Agricultural Experiment Station Journal Article No. 2984. Approved by the Director.

2 Kraft Inc., Box T, Albany MN 56307.






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Copyright © 1985 by the American Dairy Science Association ®.