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Journal of Dairy Science Vol. 68 No. 2 290-299
© 1985 by American Dairy Science Association ®
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Improved Assay Procedure for Determination of Milk-Clotting Enzymes1

Alfred Carlson and Charles G. Hill, Jr.

Department of Chemical Engineering

Norman F. Olson

Department of Food Science, and Walter V. Price Cheese Research Institute, University of Wisconsin, Madison 53706

ABSTRACT

An assay technique for quantifying milk-clotting enzymes is described. By the technique, enzyme concentrations as small as 2 x 10–5 rennet units/ml can be determined accurately in milk solutions in as little as 2 h. The technique can be used to assay for enzymes in milk and whey solutions without prior removal of casein. The technique should find application in detecting enzymes in a variety of systems commonly encountered in studies of milk and cheese products.


FOOTNOTES

1 A contribution from the Walter V. Price Cheese Research Institute.







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Copyright © 1985 by the American Dairy Science Association ®.