HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chew, B. P. Articles by Tanaka, T. S. Search for Related Content PubMed PubMed Citation Articles by Chew, B. P. Articles by Tanaka, T. S.

In Vitro Growth Inhibition of Mastitis Causing Bacteria by Phenolics and Metal Chelators^1,2,

Department of Animal Sciences, Washington State University, Pullman 99164-6320

ABSTRACT

Antimicrobial activities of three phenolic compounds and four metal chelators were tested at 0, 250, 500, and 1000 ppm in vitro against four major mastitis-causing bacteria, Streptococcus agalactiae, Staphylococcus aureus, Klebsiella pneumoniae, and Escherichia coli. Overall, butylated hydroxyanisole and tert-butylhydroquinone showed the greatest antimicrobial activity. These phenolics were bactericidal at 250 to 500 ppm against all four bacteria tested. The butylated hydroxytoluene was bactericidal against the gram-positive bacteria but was ineffective against the coliforms. At 250 ppm, disodium ethylenediaminetetraacetic acid was bactericidal against the gram-positive bacteria but much less effective against the gram-negatives. However, diethylenetriaminepentaacetic acid was more growth inhibitory than ethylenediaminetetraacetic acid against the gram-negative bacteria and especially against Escherichia coli. All other compounds were generally much less effective or ineffective against all four microorganisms. Therefore, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone, ethylenediaminetetraacetic acid, and diethylenetriaminepentaacetic acid may have practical implications in the prevention or treatment of bovine mastitis.

¹ Research supported by the Washington State University Agricultural Experiment Station, Project No. 0525. Scientific Paper No. 7005.

² Use of trade names does not imply endorsement of the product by the Washington State University Agricultural Experiment Station.

This article has been cited by other articles:

				R. M. Q. Shanks, J. L. Sargent, R. M. Martinez, M. L. Graber, and G. A. O'Toole Catheter lock solutions influence staphylococcal biofilm formation on abiotic surfaces Nephrol. Dial. Transplant., August 1, 2006; 21(8): 2247 - 2255. [Abstract] [Full Text] [PDF]