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Growth of Bifidobacteria in Milk and Preparation of Bifidobacterium infantis for a Dietary Adjunct

Department of Food Science and Technology, University of California, Davis 95616

ABSTRACT

Addition of .2% modified deMan, Rogosa, Sharp broth permitted growth of four species of Bifidobacterium in either 10 or 12% nonfat dry milk. Serial propagations of Bifidobacterium adolescentis, Bifidobacterium infantis, and Bifidobacterium longum were successful in the modified milk, but Bifidobacterium bifidum required addition of ascorbic acid. Cysteine (.05%) plus either pyruvic acid (.05%) or ascorbic acid (.2%) successfully replaced addition of the broth for Bifidobacterium longum and Bifidobacterium bifidum. Bifidobacterium infantis was stable to lyophilization, and lyophilized Bifidobacterium infantis showed no decrease of viability during storage for 4 mo at 4°C under air in a dessicator or during suspension (2 x 10⁶/ml) for at least 7 days in refrigerated pasteurized lowfat milk.