HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jeon, I. J. Articles by Hennessy, K. J. Search for Related Content PubMed Articles by Jeon, I. J. Articles by Hennessy, K. J.

Rapid Determination of Lactose and Its Hydrolyzates in Whey and Whey Permeate by High Performance Liquid Chromatography¹

Animal Sciences and Industry, Kansas State University, Manhattan 66506

ABSTRACT

A rapid method is presented for the quantitative analysis of lactose, glucose, and galactose in whey and whey permeate by high performance liquid chromatography. Samples were prepared by heating to precipitate most proteins and by eluting through a Sep-Pak^TM cartridge to remove lipids, chromophores, and residual proteins. Sugars were separated on an Altex µ-Spherogel Carbohydrate column and measured by a refractive index detector. Recovery of added carbohydrates averaged 98.0% for lactose and 101.5% for glucose and galactose. Coefficients of variation for recovery ranged from .27 to 3.20%. Reproducibility was demonstrated by our monitoring rate of enzymatic hydrolysis of lactose in whey permeate. Sample preparation time was 15 min; chromatographic separation was within 15 min.

¹ Contribution No. 83-227-J, Department of Animal Sciences and Industry, Kansas Agricultural Experiment Station, Kansas State University, Manhattan 66506.