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Journal of Dairy Science Vol. 67 No. 4 759-763
© 1984 by American Dairy Science Association ®
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Determination of Soluble Carbohydrates in Yogurts by High Performance Liquid Chromatography

C. Vidal-Valverde, C. Martin-Villa and J. Herranz

Nutrition Service, Clinica Puerta de Hierro, Centro Nacional de Investigaciones Médico-Quirúrgicas, de la Seguridad Social, Universidad Autónoma, S. Martin de Porres, 4 Madrid-35, Spain

ABSTRACT

A procedure is described that allows simultaneous identification and quantification of sucrose, lactose, glucose, galactose, and fructose by high performance liquid chromatography. A Sugar-Pak I column (Waters) at 85°C or 90°C and refractive index detector were used with double distilled water as mobile phase. The procedure was appropriate for determination of soluble sugars in fermented dairy products and has been applied to analysis of several commercial yogurts.






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Copyright © 1984 by the American Dairy Science Association ®.