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Effectiveness of Rumen-Protected Methionine for Delivering Methionine Postruminally in Dairy Cows

Research Laboratories Eastman Chemicals Division, Eastman Kodak Company, Rochester, NY 14603

C. J. Sniffen and T. V. Muscato

Department of Animal Science, Cornell University, Ithaca, NY

ABSTRACT

A methionine preparation encapsulated for protection from degradation in the rumen was studied in vitro and in vivo to determine: a) its effectiveness in delivering methionine postruminally; and b) its effect on the concentration of blood-plasmamethionine and other amino acids and on production by dairy cows. Rumen-protected methionine was 94% stable in pH 5.4 buffer, which simulated ruminal pH, and it released 94% of methionine in pH 2.9 buffer, which simulated abomasal pH. Twenty-five Holstein cows in early lactation were used to evaluate this preparation in vivo. Cows were assigned randomly to receive one of the following five concentrations of rumen-protected methionine: 0, 157, 315, 472, and 630 mg/kg feed as fed. This trial consisted of a 3-wk base period (no rumen-protected methionine fed) followed by a 3-wk dose period (treatment diets fed). Methionine concentrations in blood plasma for the five dietary rumen-protected methionine concentrations were (µg/ml): 3.4, 4.0, 4.8, 5.1, and 5.3. Corresponding concentrations for the base period were (µg/ml): 3.2, 3.4, 3.5, 3.4, and 3.4. The linear increase of methionine in plasma indicates this preparation delivered methionine postruminally. There was no effect on concentration of other plasma amino acids. The cows receiving rumen-protected methionine consumed more feed and gained more weight than did the control cows. Other production measures were unaffected.

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