HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wanger, A. R. Articles by Dunny, G. M. Search for Related Content PubMed PubMed Citation Articles by Wanger, A. R. Articles by Dunny, G. M.

Specific Agglutination of Streptococcus agalactiae from Bovine Mastitis by Casein Components of Bovine Milk

Department of Veterinary Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853

¹ Reprint requests.

ABSTRACT

Streptococcus agalactiae strains freshly isolated from bovine mastitis cases clumped within 15 min of addition of small amounts of bovine milk to a broth culture. This reaction was not observed with isolates from human infections or bovine strains that had been maintained in the laboratory for extensive periods. Intensity of the clumping response as measured by a microtiter dilution assay was highly variable. Analysis of several single colony isolates derived from one strain indicated that the clumping phenotype was genetically unstable. The clumping reaction took place in the presence of rifampicin or chloramphenicol. Milk components that caused aggregation were heat stable, relatively insensitive to proteases, and were larger than 10,000 daltons. Purified casein also induced clumping in these strains.