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In Vitro Hepatic Gluconeogenesis during Experimental Ketosis Produced in Steers by 1,3-Butanediol and Phlorizin¹

Nutritional Physiology Group, Department of Animal Science, Iowa State University, Ames 50011

ABSTRACT

Adaptations of in vitro incorporation of gluconeogenic substrates into glucose and adaptations of metabolite concentrations of liver to subcutaneous phlorizin and dietary 1,3-butanediol were examined for liver samples from dairy steers. Later, the same adaptations were examined after 6 days of feed restriction. Feeding 1,3-butanediol significantly decreased conversion of carbon-14 of lactate and propionate to glucose and to carbon dioxide. There were no changes of concentrations of hepatic glycogen or triglyceride, and increases were only minor for ß-hydroxybutyrate concentration. Both phlorizin, with or without 1,3-butanediol, and feed restriction significantly increased rates of carbon incorporation into glucose from aspartate, lactate, and propionate but did not change rates of oxidation to carbon dioxide. Phlorizin had no effect on hepatic glycogen or triglyceride concentrations, but feed restriction decreased liver glycogen and increased triglyceride concentrations. Changes associated with either phlorizin treatment or feed restriction are consistent with a decreased ratio of insolin to glucagon of blood plasma. When combined, plorizin and 1,3- butanediol seem to have some utility for developing a ketosis model.

¹ Journal Paper No. J-10980 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Projects No. 2350 and 2389. This material is based upon work supported by the US Department of Agriculture under Agreement No. SEA-CR 901-15-160. Data were taken from a dissertation submitted to Iowa State University by S. E. Mills to fulfill partly the requirements for the Ph.D. degree. A paper was presented at the 1982 Annual Meeting of the American Dairy Science Assoc, University Park, PA [J. Dairy Sci. 65(Suppl. 1):119 (Abstr.)].

² Purdue University, Department of Animal Sciences, Lilly Hall, West Lafayette, IN 47907.

³ Ralston Purina Co., Beef and Sheep Research-2AP, Checkerboard Square, St. Louis, MO 63164.