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Glycosidic Activities of Pseudomonas fluorescens on Fat-Extracted Skim Milk, Buttermilk, and Milk Fat Globule Membranes¹

Departments of Food Science and Nutrition and Biochemistry University of Missouri-Columbia, Columbia 65211

ABSTRACT

Crude enzyme of Pseudomonas fluorescens 26, a psychrotrophic microorganism, was incubated at 20°C for 2 days with skim milk, washed-cream buttermilk, and milk fat globule membrane. The first two milk fractions were fat-extracted.

Skim milk was the best substrate for glycosidase studies, because it had the highest amount of potentially hydrolyzable carbohydrates. Furthermore, it was the fraction most enzymatically degraded. Galactose was the hexose most readily removed from skim milk, as expressed in units of concentration (µ/ml). Among all carbohydrates, hydrolysis of fucose was greatest in buttermilk and milk fat globule membrane, but substrate-limitation was possible. Unlike sialic acid, which was hydrolyzed well in all fractions, hexosamines were hydrolyzed poorly, probably due to their putative position in the middle of the oligosaccharide chain or in the carbohydrate-protein linkage of glycoproteins.

¹ Contribution from the University of Missouri Experiment Station, Journal Series Number 9165.

² Department of Food Science and Nutrition.

³ Department of Biochemistry.