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Vitamin E Supplementation of High Fat Diets for Dairy Cows^{1, 2,}

Department of Dairy Science, Ohio Agricultural Research and Development Center, Wooster 44691

ABSTRACT

High fat diets ± 1 g -tocopheryl acetate per head per day were fed to 28 cows for 4 mo to determine whether dietary fat increases dietary requirement for vitamin E.

Tocopherol supplementation did not affect milk production or composition. After 4 mo there was no difference between treatment groups for oxidative stability (thiobarbituric acid test) of milk stored for 5 days with .1 ppm added copper, but milk of supplemented cows was more stable when 1 ppm copper was added. Oxidative stability was normal in the unsupplemented group.

Tocopherol concentration of blood plasma increased and selenium concentration decreased with tocopherol supplementation; activity of glutathione peroxidase was lower in plasma, whole blood, and erythrocytes of the supplemented group. Tissue integrity was unchanged by tocopherol supplementation, as estimated by erythrocyte hemolytic resistance and activities of lactic dehydrogenase and glutamic oxalacetic transaminase in blood serum. Clearance of bromosulphthalein from blood plasma was less for cows fed fat and supplemented with tocopherol than for control cows fed neither fat nor vitamin E. No indication of impaired vitamin E status was observed when 4% stabilized fat was added to the diet.

¹ Salaries and research support provided by State and Federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University. Journal Article No. 188-82.

² From a thesis presented by Paula K. Lundin in partial fulfillment of the M.S. degree, The Ohio State University, Columbus 43210.