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Effect of Large Oral and Intravenous Doses of Vitamins D₂ and D₃ on Vitamin D in Milk¹

Department of Health and Welfare, Bureau of Nutritional Science, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario K1A 0L2

M. Hidiroglou

Animal Research Centre, Agriculture Canada, Research Branch, Ottawa, Ontario K1A 0C6

ABSTRACT

Vitamins D₂ and D₃ were measured in milk after high performance liquid chromatography of unsaponifiable lipids first on a silica column and then on a reverse-phase column. Milk from cows kept indoors contained barely detectable vitamin (less than 2 IU/100 ml) predominantly in the form of vitamin D₃. When cows were given 5 or 10 million IU vitamin D₃, maximums were in milk 3 to 7 days after oral doses and up to 10 days after intravenous injections. Maximums ranged from 7 to 90 IU/100 ml. Two cows were given a mixture of 1 million IU vitamin D₂ and 1 million IU vitamin D₃ orally, and of the vitamins were maximum 2 to 3 days after the dose in blood plasma and a day later in milk. Equal amounts of the two forms of the vitamins were in milk, but vitamin D₃ in plasma was double vitamin D₂. Different mechanisms control concentrations of vitamins D₂ and D₃ in blood and milk after large doses.

¹ ARC Contribution No. 1099.