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Journal of Dairy Science Vol. 66 No. 6 1232-1236
© 1983 by American Dairy Science Association ®
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Isocitrate Lyase Activity in Lactobacillus murinus CNRZ 313

M. C. Albizzatti de Rivadeneira1, M. C. Manca de Nadra2, A. A. Pesce de Ruiz Holgado2 and G. Oliver2

Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán
Centre de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000 S.M. de Tucumán, República Argentina

ABSTRACT

Isocitrate lyase activity in Lactobacillus murinus CNRZ 313 was demonstrated. In cell-free extracts partially purified by ammonium sulphate precipitation, maximum activity was at 37°C, pH 7.9, in the presence of 16 mM glutathione and 2.4 mM cysteine. The extracts exhibited an apparent Michaelis constant 1.17 x 10–2 M. Preincubation of the extract for 20 to 60 min at 40°C stimulated enzymatic activity.

FOOTNOTES

1 Institute Miguel Lillo, Universidad Nacional de Tucumán.

2 Career Investigator Consejo Nacional de Investigaciones Cientificas y Técnicas de la República Argentina.






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Copyright © 1983 by the American Dairy Science Association ®.