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Immunological Approach to Location of -Casein in the Casein Micelle by Electron Microscopy

Agricultural Research Service, US Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118

ABSTRACT

The colloidal stability of skim milk is due to -casein in casein micelles; thus, the location of this protein within these complexes has been the subject of speculation. Various models have placed -casein at the surface, in the interior, or distributed uniformly in the casein micelle. By a ferritin-labeled double-antibody technique coupled with electron microscopy, location of -casein has been related to casein micelle size. Casein micelles with -casein located predominantly at the periphery of the micelle have diameters of 1420 ± 320 Å whereas micelles with -casein located uniformly throughout have diameters of 920 ± 220 Å. These results are in accord with the inverse relationship between micelle size and -casein content and support the model of Slattery (19).