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Solubilization of Sulfhydryl Oxidase, A Bovine Skim Milk Membrane Enzyme¹

Departments of Biochemistry and Food Science, North Carolina State University, Raleigh 27650

ABSTRACT

Sulfhydryl oxidase of bovine milk was prepared by gel chromatography of crude skim milk membranes obtained by ammonium sulfate fractionation of whey. A variety of detergents were screened for their potential effectiveness as enzyme-solubilizing agents. Anionic detergents, such as sodium dodecyl sulfate and sodium octyl sulfate, inactivated the enzyme concomitantly with solubilization. Nonionic detergents, however, were generally effective in solubilizing sulfhydryl oxidase near their critical micelle concentrations. Polyoxyethylene-9-lauryl ether and ß-octyl-D-glucoside afforded the best solubilization of sulfhydryl oxidase among the detergents tested; enzymic activity was retained fully at detergent concentrations producing over 80% solubilization.

¹ Paper 8011 of the Journal Series of the North Carolina Agricultural Research Service. The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products.

² National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, NIH, Bethesda, MD 20205.

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