JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 65 No. 8 1401-1407
© 1982 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Azuma, N.
Right arrow Articles by Yamauchi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azuma, N.
Right arrow Articles by Yamauchi, K.

Identification of a Calcium-Binding Protein in Human Milk as {alpha}-Lactalbumin

Norihiro Azuma, Shuichi Kaminogawa, Hiromasa Kobayashi and Kunio Yamauchi

Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

ABSTRACT

Calcium-binding activity of proteins in human milk was surveyed by Chelex-100 assay. Two calcium-binding proteins besides caseins were found by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl Sephadex. Results of the amino acid analysis and immuno-double diffusion confirmed that these two components were the same protein and identical with {alpha}-lactalbumin.

Studies about the effect of ethyl-diaminetetraacctate on electrophoretic (Disc-polyacrylamide-gel electrophoresis) mobility and on thermal stability suggested that human {alpha}-lactalbumin is a calcium metalloprotein. One mole of human {alpha}-lactalbumin appeared to bind one mole of calcium ions, and the formation constant for the calcium-human {alpha}-lactalbumin complex was 1.0 x 105 M-1.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the American Dairy Science Association ®.