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Journal of Dairy Science Vol. 65 No. 8 1394-1400
© 1982 by American Dairy Science Association ®
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Separation of Carbohydrates in Dairy Products by High Performance Liquid Chromatography1

M. L. Richmond2, D. L. Barfuss, B. R. Harte2, J. I. Gray and C. M. Stine

Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824

ABSTRACT

A simple procedure is described for determination of carbohydrates commonly in dairy products. HPX—87 carbohydrate column and refractive index detector were used to resolve lactose, glucose, and galactose in only 8 min. An Aminex MicroguardTM Anion/OH cartridge was used to remove interfering acids and milk salts from dairy foods. The problem of resolving lactose from sucrose on resin based systems is discussed. Applications include determination of carbohydrates in yogurt and cultured buttermilk.

FOOTNOTES

1 Michigan Agricultural Experiment Station Journal Article Number 10076.

2 School of Packaging, Michigan State University.






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Copyright © 1982 by the American Dairy Science Association ®.