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Rapid and Specific Isolation of Radioactive Glucose from Biological Samples¹

Nutritional Physiology Group, Department of Animal Science, Iowa State University, Ames 50011

ABSTRACT

An easy, reliable, and specific ion-exchange method is presented for isolating glucose for specific radioactivity determinations from both blood plasma and buffered in vitro incubation media. The use of a glucose binding resin (borate-charged anion resin) combines speed of ion exchange with specificity of derivative formation. Glucose specific radioactivities, determined by ion exchange on proteinfree filtrates of plasma containing [carbon-14] glucose, show excellent agreement with those from the popular glucose pentaacetate derivative method and are less variable. Carry-over of labeled acetate, propionate, lactate, glyoxylate, alanine, aspartate, or glutamate into the glucose fraction is less than .2%. Glycerol carry-over is 1.2%. Glucose recovery is increased about three times that of the glucose pentaacettate derivative method and averaged 94% from plasma filtrates.

¹ Journal Paper No. J-9995 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Projects No. 2186 and 2389. Supported in part by funds provided by PHS/NIH Grant AM 10706 and by SEA-CR grant 901-15-160.

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