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Department of Animal Sciences, Washington State University, Pullman 99164
ABSTRACT
This study investigated the association of selenium with ejaculated bovine spermatozoa. Over 75% of the radioactive selenium-75 was released after 30 min of incubation in 2 X 10-3 dithiothreitol. Of the selenium-75 released by dithiothreitol, 85% was associated with spermatozoal protein. Protein containing selenium-75 was found predominantly in a single band after polyacrylamide gel electrophoresis. Molecular weight was approximately 21,500 daltons.
1 Scientific Paper No. 5683, College of Agriculture Research Center.
2 Supported in part by Select Sires Inc. and Agriculture Research Center, Project 0237.
3 Dairy Breeding Research Center, The Pennsylvania State University, University Park, PA 16802.
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