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Single Radial Immunodiffusion Analysis for Quantitation of Colostral Immunoglobulin Concentration¹

Department of Animal Sciences, The University of Arizona, Tucson 85721

ABSTRACT

Relative accuracy of the single radial immunodiffusion technique to measure immunoglobulin concentration of colostral preparations (whey, whole, or fat-free) has been assesed. Fresh colostrum samples were analyzed for major constituents. Gammaglobulin as a standard was compared to total immunoglobulin concentration derived from single radial immundiffusion analysis of colostral preparations with no differences except between standard and whey. Differences were in part from either enhancement or interference of immunoglobulin diffusion by colostral constituents. Removal of casein and fat during whey preparation caused a concentrating effect upon immunoglobulin constituents resulting in exaggerated precipitin rings. Whey has produced unreliable results; therefore, whole colostrum is recommended for single radial immunodiffusion analysis.

¹ Journal paper 3324 of the Arizona Agricultural Experiment Station.

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