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Journal of Dairy Science Vol. 64 No. 11 2192-2196
© 1981 by American Dairy Science Association ®
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Effect of Amino Acid Concentration on Orotic Acid Production

F. R. Ehle2, J. L. Robinson, J. H. Clark and C. R. Baumrucker

Department of Dairy Science, University of Illinois, Urbana 61801

ABSTRACT

Investigations were to determine if bovine mammary tissue could synthesize orotate and to assess the influence of amino acids on production of orotate. Mammary tissue from 6 Holstein cows was obtained immediately after slaughter. Tissue slices were incubated in either a modified Krebs-Ringer bicarbonate buffer, the same medium containing a physiological concentration of amino acids, or a third medium containing a fourfold concentration of the amino acid mixture. In all experiments, tissue slices were preincubated in the physiological amino acid medium for 5, 10, and 15 min (30 total min) with fresh medium changes after each incubation interval. Tissue slices then were incubated with one of the three treatment media for three consecutive 20-min periods. Tissue slices incubated in the physiological amino acid medium for a total of 90 min synthesized 28.5 µg orotate/g wet tissue after correction for the initial tissue contribution. Orotate secreted by the tissue slices increased as concentration of amino acids increased. Lactose released into the medium was not affected by amino acid concentration. Bovine mammary tissue can synthesize substantial amounts of orotate, and tissue syndiesis of orotate but not lactose increased as amino acid concentration increased.

FOOTNOTES

1 Supported in part by the Illinois Agricultural Experiment Station and HEW PHS FD 00849.

2 Department of Animal Science, University of Minnesota, St. Paul MN 55108.






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Copyright © 1981 by the American Dairy Science Association ®.