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Animal Research Institute, Research Branch, Agriculture Canada, Ottawa, Ontario K1A OC6
ABSTRACT
Blood samples from 12 Shorthorn heifers, fed a grass silage ration and housed indoors or outdoors, were assayed for 25-hydroxyvitamin D during 9 mo. There was more in plasma during the summer from the outdoor group, reflecting a greater exposure to ultraviolet light and dermal synthesis of vitamin D, the precursor of 25-hydroxyvitamin D. In another experiment, assays were in four groups of six heifers each; 1) control, 2) single oral dose of 1,000,000 IU vitamin D3, 3) injected intramuscularly with 1,000,000 IU of vitamin D3, and 4) free access to a mineral mixture containing 32,000 IU vitamin D3/kg. In all groups, 2 5-hydroxy vitamin D was higher in plasma in summer than in winter. All heifers given vitamin D had more 25-hydroxyvitamin D in plasma during winter than controls. Animals injected with vitamin D had more 25-hydroxyvitamin D in plasma during part of the winter than those on the other treatments with vitamin D.
1 Contribution No. 824, Animal Research Institute, Ottawa, Ontario K1A OC6.
2 Kapuskasing Experimental Farm, Kapuskasing, Ontario P5N 2X9.
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