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Determination of Lactose in Milk Products by High-Performance Liquid Chromatography^{1 ,2,}

Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824

ABSTRACT

A method to determine lactose in dairy products by high-performance liquid chromatography is described. Samples suitable for assay were prepared from the liquid state by deproteinization with trichloroacetic acid (6% wt/vol, final concentration). Lactose was separated by injection of the deproteinized sample onto a µBondapak/Carbohydrate®³ column and isocratic elution with an acetonitrile/water mobile phase. Detection was with a differential refractometer. Sequential injection of a lactose standard and sample, addition of a standard quantity of lactose to the sample, and addition of an internal standard (xylose) to the sample were techniques for quantitation.

The validity of high-performance liquid chromatography as a method for determining lactose concentration in milk products was supported by recovery experiments and comparison of results with those by a phenol-sulfuric acid, colorimetric assay.

¹ Michigan Agricultural Experiment Station Journal Article Number 8773.

² Supported in part by a grant from Dairy Research, Incorporated (DRINC).