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Sister Chromatid Exchange in Chromosomes of Cattle (Bos taurus)¹

Istituto di Produzione animale, Universita degli studi di Napoli, Portici Italy
and Department of Animal Science, University of Minnesota, St. Paul 55108

ABSTRACT

Lymphocytes from clinically healthy Holstein animals (8 males and 23 females) were cultured in media containing 5-bromodeoxyuridine (10 /µg/ml) for two cycles of deoxyribonucleic acid replication (48 h). The exchange of sister chromatids per cell varied from 1 to 16 with a mean of 5.4 and a standard deviation of 2.1 in 603 differentially stained metaphase chromosome spreads. The major fraction of exchanges in the X chromosome were located in the region of the subcentromeric G negative bands ql and q2. When the cells were pulse labeled with tritium labeled thymidine (l/µCi/ml) for the last 6 h of the cell cycle, the rate of exchange was higher in the late replicating X chromosome (facultative hetero-chromatin) in comparison to its homo-logue (active X). This study characterizes the yield of sister chromatid exchange in lymphocytes exposed to a given amount of 5-bromodeoxyuridine in a population of normal individuals so that it may be used as a standard for 1) a diagnostic tool in pathological conditions and 2) as an assay of chromosome stability in relation to environmental hazards.

¹ Contribution No. 10457 Scientific Journal Series of the Minnesota Agricultural Experiment Station.

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