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Journal of Dairy Science Vol. 62 No. 3 502-504
© 1979 by American Dairy Science Association ®
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Rapid Separation of Whey Proteins by Cellulose Acetate Electrophoresis

J. W. Bell and W. K. Stone

Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg 24061

ABSTRACT

A rapid electrophoretic method was developed for separating whey proteins on cellulose acetate strips. Tris-barbital-sodium barbital buffer was used at pH 8.6 (ionic strength .097) with a constant current of 225 V for 1 h. Densitometer tracings showed excellent resolution of five peaks. The method can be used for ß-lactoglobulin typing of milk and for monitoring heat denaturation of whey proteins.






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Copyright © 1979 by the American Dairy Science Association ®.