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Sensitive Assay for Proteases in Sterile Milk¹

Ohio Agricultural Research and Development Center and The Ohio State University, Columbus 43210

ABSTRACT

A sensitive assay for protease activity based on the reaction of primary amino groups of trichloroacetic acid-soluble peptides and amino acids with fluorescamine was applied to sterile milk. The assay was linear in the range of 2 to 50 nmol of amino groups per aliquot. The assay is suitable for determining proteolytic activity in sterile milk products and for determining protease activity in general.

¹ Journal Article No. 6679 Ohio Agricultural Research and Development Center.

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