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Lignin as Determined by Oxidation with Sodium Chlorite and a Comparison with Permanganate Lignin¹

Michigan State University, East Lancing 48824

ABSTRACT

Sodium chlorite oxidation, a technique of plant research for 30 yr, is of the phenyl ring of lignin without removal of polysaccharides. Our objective was to apply this technique for forages and feeds to compare with permanganate lignin. Fifteen feedstuffs were selected to represent a broad section of fibrous substrates. Substrates were dried at 60 C for 48 h and ground in a Wiley Mill (1 mm screen). One-gram samples were dispersed in a .5% ammonium oxalate, boiled for 2 h, and filtered. The extracted fiber was resuspended in 1% acetic acid at 70 C, sodium chlorite (1.25 g) was added, and lignin was oxidized for a maximum of 45 min. Oxidation was stopped by adding ascorbic acid, and the suspension was filtered and dried at 60 C for 4 h. The difference in weight was defined as sodium chlorite lignin. Permanganate lignin and sodium chlorite lignin for the substrates were: ladino clover 4.94, 5.95; red clover 3.80, 5.02; Kentucky bluegrass 5.56, 6.06; Reed canary grass, full bloom 5.85, 5.35; Reed canary grass, 2nd cutting 5.33, 6.06; bromegrass 5.54, 5.54; orchardgrass 4.68, 6.73; tall fescue 3.13, 7.29; Reed foxtail 4.46, 4.99; alfalfa 6.21, 5.66; wheat straw 12.44, 8.72; elodea 4.09, 3.65; cattle manure 9.61, 13.07; birdsfoot trefoil 8.73, 6.76; and wheat middlings 4.63, 7.09. Differences in lignin values were considerable with the various substrates, but sodium chlorite lignin values generally tended to be higher than permanganate lignin.

¹ Michigan Agricultural Experiment Station Journal Article No. 8392.

² Agricultural Fermentation and Nutrition Laboratory, Department of Animal Husbandry.

³ Ruminant Nutrition Laboratory, Department of Animal Husbandry.

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