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Selective Solubilization of Marker Enzymes of Buffalo Milk Fat Globule Membrane by Detergents

National Dairy Research Institute, Karnal 132001, India

ABSTRACT

Activities of alkaline phosphomonoesterase, xanthine oxidase, 5'-nucleotidase, and adenosine triphosphatase associated with buffalo milk fat globule membrane were assessed in the presence of detergents Triton X-100, Tween-20, and deoxycholate. All detergents enhanced the enzyme activities, except adenosine triphosphatase, which was inhibited. After treatment of the membrane material by any of the detergents and subsequent centrifugation at 100,000 x g for 1 h, the recovery of alkaline phosphomono-esterase, xanthine oxidase, and 5'-nucleotidase activities in the supernatant varied. Deoxycholate treatment of the membrane material resulted in maximum recovery of these enzymes in the supernatant, but sodium dodecyl sulphate at 2 mM inhibited all the enzymes.