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A Method for Rapid Determination of Lactose^1,2,

Nutrition and Food Science Department, The University of Arizona, Tucson 85721

ABSTRACT

A simple, precise, and accurate colorimetric method for estimation of lactose in fluid milk and whey is described. The color development is based on the combined action of phenol, sodium hydroxide, picric acid, and sodium bisulfite with lactose. The method can be completed in less than 30 min. There was little difference between results by the method and the A.O.A.C. procedure. This method has advantages over other methods which require extensive time, sophisticated equipment, expensive or corrosive re-agents, considerable glassware, or appreciable analytic skill.

¹ Journal paper 2781 of the Arizona Agriculture Experiment Station.

² Supported in part by Grant AID/la-145 (Brazil Contract).

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