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Journal of Dairy Science Vol. 61 No. 2 168-175
© 1978 by American Dairy Science Association ®
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Transport of Bovine Milk Xanthine Oxidase into Mammary Glands of the Rat1

Barbara A. Blakistone2, Edward C. Sisler3 and Leonard W. Aurand

Department of Food Science and Department of Biochemistry, North Carolina State University Raleigh, NC 27607

ABSTRACT

This research investigated transport of bovine milk xanthine oxidase into mammary glands of the lactating rat. Transport capability suggested an exogenous, nonmammary, source for the enzyme. Five lactating rats were injected intracardially with 100 µg of purified iodine-125 labeled xanthine oxidase and five were injected with 100 µg of the enzyme unpurified. Four hours later the rodents were hand-milked, and radiation was confirmed in all samples by liquid scintillation counting. Counts were recorded per volume of milk and the percentage radiation was computed. Autoradiographs of the rats indicated radiation almost exclusively associated with the mammary glands. Greatest concentration of radioactivity was in the micellar casein fraction of milk, and a compound of high molecular weight, presumably [iodine-125] xanthine oxidase, was confirmed by gel filtration of the casein. Results suggest that the compound was transported into the mammary glands. The degree of transport was dependent upon the stage of lactation.

FOOTNOTES

1 Paper No. 5313 of the Journal Series of North Carolina Agricultural Experiment Station. The use of tradenames in this publication does not imply endorsement by the North Carolina Agricultural Experiment Station nor criticism of similar ones not mentioned.

2 This work represents part of the requirement for the degree of Master of Science at North Carolina State University.

3 Department of Biochemistry.






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Copyright © 1978 by the American Dairy Science Association ®.