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Interrelationship between Apoproteins of Very Low Density Lipoprotein and Other Serum Lipoproteins in Lactating Goats

National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT, England

ABSTRACT

Lipoproteins of goat serum were labeled in vivo by intravenous injection of tritium labeled DL-lysine monohydrochloride. The specific radioactivity of the very low density plus intermediate density fractions reached a maximum at 1.2 h, declined sharply until about 9 h, and then more slowly. The specific radioactivities of the low density and high density lipoproteins reached lower maxima at 6.5 h and 8.5 h, respectively.

When iodine-125-labeled very low density lipoprotein of goats was incubated in vitro with buffer there was a transfer of 11% of radioactivity, chiefly to the intermediate and low density lipoproteins, but on incubation in serum 29% of the radioactivity was distributed equally among the other lipoproteins. On gradient gel electrophoresis of the apo-lipoproteins, the slowest migrating zones in the very low density, intermediate density, and low density lipoproteins were the most radioactive whereas over 90% of the radioactivity in the apoproteins of the high density lipoprotein was in two zones, one of which was at the origin.

The results are consistent with the formation of low density lipoprotein from very low density lipoprotein via the intermediate density lipoprotein and with a transfer of apoproteins between very low density and high density lipoproteins.

¹ Dr. Tamir participated in the in vitro experiments while on sabbatical leave from Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan, Israel.