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Hydrolysis of Lactose by Microbial ß-Galactosidases. Formation of Oligosaccharides with Special Reference to 2-0-ß-D-galactopyranosyl-D-glucose

Laboratory of Animal Products, Technology College of Agriculture, Tohoku University, Sendai, Japan

ABSTRACT

The transgalactosylation of lactose by Saccharomyces fragilis ß-galactosidase and Aspergillus niger ß-galactosidase was examined. With S. fragilis ß-galactosidase, twelve oligosaccharides including five disaccharides were detected on paper chromatograms. With A. niger ß-galactosidase, ten oligosaccharides were formed. The disaccharides formed by A. niger ß-galactosidase were similar to those by S. fragilis ß-galactosidase.

These disaccharides were isolated by preparative paper chromatography and their structures were explored by gas-liquid chromatography of their methylated methanolyzates. Comparisons of mobility and color of the spots on paper electrophoretograms were also carried out. The disaccharides proved to be 2-0-ß-D-galactopyranosyl-D-glucose, 3-0-ß-D-galactopyranosyl-D-glucose, 6-0-ß-D-galactopyranosyl-D-glucose, 3-0-ß-D-galactopyranosyl-D-galactose, and 6-0-ß-D-galactopyranosyl-D-galactose.