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Journal of Dairy Science Vol. 60 No. 8 1301-1303
© 1977 by American Dairy Science Association ®
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Survival of Mouse Embryos after Freezing in Medium Containing Dimethylsulphoxide and Yolk Extract

P. S. Fiser and J. W. Macpherson

Department of Animal and Poultry Science, Univesity of Guelph, Guelph, Ontario N1G 2W1

ABSTRACT

The ability of the cryoprotective solution containing dimethylsulphoxide in combination with yolk extract (DYG 550) to protect mouse embryos at ultra low temperatures is reported. Of the embryos 46.1% frozen to –196 C at various rates formed blastocysts in vitro compared to 41.2% and 23.2% survivals protected by 1M dimethylsulphoxide and 1M glycerol.






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Copyright © 1977 by the American Dairy Science Association ®.