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Journal of Dairy Science Vol. 60 No. 6 889-895
© 1977 by American Dairy Science Association ®
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Lactational Events Related to Glucocorticoid Binding in Bovine Mammary Tissue1

R. C. Gorewit2 and H. A. Tucker

Animal Reproduction Laboratory, Department of Dairy Science, Michigan State University, East Lansing 48824

ABSTRACT

Various synthetic glucocorticoids reduced binding of tritiated Cortisol and tritiated dexamethasone to 700 x g supernatant and precipitate fractions of mammary tissue slices from lactating cows. Unlabeled progesterone, testosterone, and 17ß-estradiol had no effect on tritiated glucocorticoid binding in mammary tissue slices. Cortexelone, Cortisol, triamcinolone, and dexamethasone inhibited [carbon-14] glucose incorporation into mammary tissue slices from lactating cows in a dose response relationship. Glucocorticoid binding to 700 x g supernatant and precipitate fractions of mammary tissue was correlated with the ability of glucocorticoids to reduce [carbon-14] glucose uptake into 700 x g supernatant fractions of mammary tissue slices from lactating cows. In experiments designed to measure mammary uptake of glucocorticoids, the differences in concentration of total glucocorticoids between external pudic artery and mammary vein were greatest at 6 (5.8 ng/ml) and 12 (2.9 ng/ml) min after the start of milking. These intervals correspond to the times when glucocorticoid concentrations in serum were maximal after application of the milking stimulus. We conclude that glucocorticoid uptake and binding are associated with lactational events.

FOOTNOTES

1 Published with the approval of the Director of the Michigan Agriculture Experiment Station as Journal No. 7846. This Research was supported in part by USPHS Grant No. HD-05750 and FD-00706.

2 Department of Animal Science, Cornell University, Ithaca, NY 14853.






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Copyright © 1977 by the American Dairy Science Association ®.