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Preparation and Storage of Concentrated Cultures of Lactic Streptococci

Department of Food Science, North Carolina State University, Raleigh 27607

ABSTRACT

INTRODUCTION

Concentrated starter cultures are highly concentrated suspensions of the bacteria which make up a normal or conventional starter culture. The bacterial cells for preparing concentrates are grown under closely controlled conditions in a liquid medium, concentrated into a small volume by centrifugation, and then placed in frozen storage. They represent one of the more recent major advances in starter culture technology and now are used routinely in the manufacture of many cultured dairy products. The development of concentrated cultures for inoculating bulk starters or the product vat directly has eliminated much of the time, drudgery, and dangers customarily involved in preparing and maintaining starter cultures in the dairy plant. The dairy processor no longer has to maintain stock cultures by regular subculture nor does he have to build up sufficient volumes of culture by successive transfers in order to inoculate the product to be cultured. This has eliminated many chances for contamination of the culture by undesirable bacteria or bacteriophage.