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Comparison of Two Methods for Analysis of Aflatoxin M₁ in Milk¹

Department of Dairy Science, Clemson University, Clemson, SC 29631

ABSTRACT

The cellulose chromatography method of the Association of Official Analytical Chemists for determination of Aflatoxin M₁ was compared with a two-dimensional thin layer chromatography procedure. The minimum detectable Aflatoxin M₁ by the Official procedure was approximately .01 µg/kg while the thin layer chromatography procedure has a claimed sensitivity of .004 µg/kg. Analyses of milk samples by thin layer chromatography agreed with results of the Official procedure in the range of .01 to .25 µg/liter. Each method consistently yielded recoveries greater than 87%, and there was no significant difference between means or variances of each method. The thin layer chromatography method took less time than the Official method and showed a reproducible lower threshold sensitivity of .005 µg/liter.

¹ Technical Contribution No. 1365, South Carolina Experiment Station. Published by permission of the Director.