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Journal of Dairy Science Vol. 60 No. 12 1987-1990
© 1977 by American Dairy Science Association ®
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Hexose Shunt Dehydrogenase Activity in Leukocytes Isolated from Bovine Milk1

Carl Ritter, Allen Conti and G. E. Morse

Department of Animal Biology and Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street Philadelphia, PA 19174

ABSTRACT

A spectrophotometric method for measuring small changes in light absorption at specific wavelengths from highly turbid suspensions of biological material (dual-beam spectrophotometry) has been used to measure glucose-6-phosphate oxidation and nicotinamide adenine dinucleotide phosphate (oxidized) reduction catalyzed by lysates of milk leukocytes. This reduction was correlated .84 with cellular protein concentration and, therefore, with cell concentration.

FOOTNOTES

1 Supported by summer fellowships in 1974 and 1975 from Xienta Corp., Bernville, PA.






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Copyright © 1977 by the American Dairy Science Association ®.