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Measurement of 25-Hydroxycholecalciferol in the Bovine¹

Department of Dairy Science, Ohio Agricultural Research and Development Center, Wooster 44691

ABSTRACT

Bovine plasma 25-hydroxycholecal-ciferol was estimated by rapid protein binding assay. Serum-binding proteins in the assay were from rats deficient in vitamin D and from normal rats; no differences existed in standard curves. Nanograms 25-hydroxycholecalciferol in eight cows averaged 45.5 ± 12.2 ml of plasma. Results from the rapid protein binding assay compared favorably (>10% deviation) with values from a 25-hydroxycholecalciferol assay involving high pressure liquid chromatography.

¹ Approved for publication as Journal Article No. 37–76, Ohio Agricultural Research and Development Center, Wooster 44691.

² Data in this paper were presented to the Graduate Faculty of The Ohio State University in partial fulfillment of the requirement for the degree Master of Science (March 1976).

³ Department of Nutrition, University of Guelph, Guelph, Ontario, Canada.