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Journal of Dairy Science Vol. 59 No. 8 1409-1413
© 1976 by American Dairy Science Association ®
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Relative Activation of Milk Lipoprotein Lipase by Serum of Cows Fed Varying Amounts of Fat1 ,2,

Dennis M. Super3, D. L. Palmquist and F. L. Schanbacher

Department of Dairy Science, Ohio Agricultural Research and Development Center, Wooster 44691

ABSTRACT

A routine laboratory assay to evaluate relative concentrations of lipoprotein lipase activator (apo C-II) in cow serum was developed. The assay was linear for at least 120 min after an initial, unexplained, lag time of 13 to 15 min. Half-maximal activation was in the range of 1 to 2% serum in the assay. Inhibition of activation was indicated at high amounts (10%) of serum. Activation from plasma was half that from serum, presumably caused by an increase in substrate Km in the presence of heparin. Use of glyceryl tri[9,10-3 H] oleate yielded excessively high blanks; [2-3 H] glyceryl triolein is suggested for routine assay. Relative amounts of activator were not different between dry and lactating cows fed "conventional" diets. Activator concentration increased linearly with increasing dietary fat and was related to concentration of total lipid in plasma. The assay may provide a useful adjunct in studies on lipoprotein metabolism.

FOOTNOTES

1 Approved as Journal Article No. 15–76, Ohio Agricultural Research and Development Center, Wooster 44691.

2 An independent study project of the senior author as partial fulfillment of the B.A. degree in Biochemistry, Ashland College, Ashland, OH, May, 1975.

3 College of Medicine, The Ohio State University, Columbus 43210.






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Copyright © 1976 by the American Dairy Science Association ®.