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Numerical Relationship between Strains in Frozen Concentrates of Lactic Streptococcal Starter Cultures¹

Department of Microbiology, Oregon State University, Corvallis, 97331

ABSTRACT

Strain dominance was revealed in two multiple strain lactic streptococcal starter concentrates by addition of phages specific for the component strains. Both strains showed clear dominance when tested 3 to 4 wk after freezing and storage at –20 C. In one starter, dominance persisted throughout 10 mo of storage. In the second starter, dominance decreased over 8 mo of storage, and the lag phase increased from 1 to 4 h. Plate counts showed only a two-fold difference in cell numbers between uninfected and phage-infected dominant strain cultures. Differential survival and recovery from freezing are suggested as factors in production and alteration of the dominance pattern.

¹ Technical Paper No. 4109 Oregon Agricultural Experiment Station.