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Journal of Dairy Science Vol. 59 No. 7 1340-1341
© 1976 by American Dairy Science Association ®
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Synthesis of Ruminal Microbial Protein and Volatile Fatty Acid Production In Vitro

J. Harmeyer, H. Martens and M. A. Naga1

Institute of Physiology, Veterinary Medicine, 3 Hannover, Federal Republic of Germany

ABSTRACT

Growth of a mixed ruminal population taken from a sheep on a protein-free (all urea) purified diet was estimated by an in vitro fermentation technique including precipitation of mircrobial protein by trichloroacetic acid. Volatile fatty acid production in vitro was determined, and the associated adenosine triphophate was estimated as moles volatile fatty acids x 2.4. On this basis, the quantity of microbial protein synthesized per mole of adenosine triphosphate increased at higher microbial growth rates.

FOOTNOTES

1 Faculty of Agriculture, University of Alexandria, Alexandria, Egypt.






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