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Limiting Amino Acid for Protein Synthesis with Mammary Cells in Tissue Culture¹

Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061

ABSTRACT

To identify the limiting amino acid in the minimal essential medium as published by Eagle (Science 130:432, 1959) for milk protein synthesis in rat mammary cells in tissue culture, two different experimental approaches were used. The first study involved the reduction of amino acids singly from the total amino acid complement of the medium for milk protein synthesis. The second study was to investigate the effect on milk protein synthesis of single amino acid addition to the basic complement of amino acids. Order of limiting amino acids was lysine (first) and possibly methionine, valine, or arginine (second).

¹ Approved for publication by the Director, Division of Animal and Veterinary Sciences. Appreciation is expressed to the William M. Hackman Foundation and to E. I. duPont de Nemours and Company for partial support of this research.