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Comparative Production of ß-Lactoglobulin and Orotic Acid with Lactose in Bovine Mammary Cell Cultures: Effects of Cell Density and Constituent Inhibition¹

Department of Dairy Science, University of Illinois, Urbana 61801

ABSTRACT

Abilities to accumulate ß-lactoglobulin and orotic acid were compared to lactose in dispersed cell cultures of lactating bovine mammary tissue. The inverse of the amount accumulated of each milk constituent at a given time in the culture medium was a linear function of the inverse of the cell density. The amount of lactose had no effect on its own subsequent accumulation, but added orotic acid and ß-lactoglobulin inhibited their own production. The accumulation of certain milk constituents in the culture medium is a factor in the expression and loss of normal function in the in vitro cultures which may be related to the observed effects of milk accumulation in vivo on the rate of milk synthesis and mammary involution.

¹ Supported by Contract NOl-CB-23856 of the National Cancer Institute, National Institutes of Health, and Hatch Projects 35-320 and 35-351 of the Illinois Agricultural Experiment Station.