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Determination of -Lactalbumin in Human Milk

Institute of Nutrition, University of Uppsala, Box 551 S-751 22 Uppsala 1, Sweden

ABSTRACT

-Lactalbumin is a major protein in human milk and of considerable nutritional importance to the infant. However, there are discrepancies in the literature on content of this protein in human milk, which indicate a need for a method that would permit estimation from a large number of samples. The technique of immunodiffusion was adopted for human -lactalbumin. Purification of -lactalbumin for production of antigen is described as is the statistical evaluation of the method. The -lactalbumin contents of pooled human milk samples as well as of the milk from one another during 2 mo of lactation were analyzed. The -lactalbumin content of human milk showed considerable variation. This variation and its relation to factors such as length of lactation, and nutritional status of the mother should be studied further.