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Acid-Catalyzed Hydrolysis of Lactose in Whey and Aqueous Solutions

Department of Food Science and Technology, University of California, Davis 95616

ABSTRACT

Lactose in fresh cottage cheese whey and aqueous solutions was successfully hydrolyzed in .5, 1, 2, and 3 Normal hydrochloric acid at 50, 60 and 70 C. First-order rate constants and half-times were calculated from glucose released by hydrolysis. Rates in both whey and aqueous lactose solutions were comparable at 50 and 60 C whereas at 70 C whey lactose rates appeared consistently lower than aqueous lactose rates (by approximately 10 to 20%). This was attributed to loss of free glucose through the browning reaction. Undesirable side reactions were minimized by conducting the hydrolysis at temperatures well below 100 C. The data demonstrate the feasibility of using mineral acid for whey lactose hydrolysis.

Hydrolysis of lactose in whey (both acid and sweet) and aqueous solutions was unsuccessful at pH 4.7 and 90 C being less than 1% after 96 h of heat treatment. Hydrolysis was also insignificant in acid whey and aqueous lactose solutions containing 5 and 10% lactic acid after treatment for 150 h at 60 C.

This article has been cited by other articles:

				A. Cote, W. A. Brown, D. Cameron, and G. P. van Walsum Hydrolysis of Lactose in Whey Permeate for Subsequent Fermentation to Ethanol J Dairy Sci, June 1, 2004; 87(6): 1608 - 1620. [Abstract] [Full Text] [PDF]