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-CaseinEastern Regional Research Center1, Philadelphia, PA 19118
ABSTRACT
The action of rennin on
-casein was studied as a function of time using several methods to measure activity. The first indication of rennin cleavage of
-casein is precipitability in .1 M acetate buffer at pH 5.2 and 5 C. A longer exposure to rennin is required to alter
-casein so that it forms a precipitate with calcium ions and loses its ability to stabilize
s-casein. The least sensitive indication of rennin activity is measurement of nitrogen soluble in 2% trichloroacetic acid. Electrophoresis experiments showed that these methods detect various stages in the conversion of
-casein to para-
-casein.
1 Agricultural Research Service, United States Department of Agriculture.
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