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Plasma Estradiol in Holstein and Beef Cows During the Periparturient Period¹

² Department of Veterinary Medicine and Surgery
and³ Agricultural Experiment Station, University of Missouri, Columbia 65201

ABSTRACT

Plasma estradiol, measured by radio-immunoassay, was compared in Holstein and beef cows during the periparturient period. A disposable Celite column was used for separation of estrogens. The antibody-estrogen complex was separated by charcoal and centrifugation. Circulating plasma estradiol was higher in 11 Holstein than in 9 beef cows prior to parturition. For Holstein cows, among nonsignificant differences, highest mean concentrations were 580 pg/ml on the day before parturition and 440 pg/ml on the day of parturition. Beef cows reached a peak of 538 pg/ml on the day of parturition. Concentrations rapidly and similarly decreased in both groups following parturition.

¹ Contribution from the Missouri Agricultural Experiment Station. Journal Series No. 6703. Approved by the Director. This work was supported in part from Veterinary Medicine Research Council Grant 329.

⁴ Present address: Department of Veterinary Clinical Sciences, University of Minnesota, St. Paul 55101.