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Journal of Dairy Science Vol. 57 No. 9 1083-1085
© 1974 by American Dairy Science Association ®
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Trace Constituents in Whey. Isolation and Identification of Amines

J. L. Weihrauch and D. P. Schwartz

Dairy Products Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Philadelphia, PA 191181

ABSTRACT

Primary and secondary amines were isolated from whey as their 2,4-dinitrophenyl derivatives, separated by thin-layer partition chromatography, and identified by gas chromatography-mass spectrometry. Examination of raw and pasteurized sweet whey, reconstituted spray dried whey powder, and protein-free ultrafiltration permeate of sweet and acid wheys revealed the same qualitative distribution of amines. Amines identified were methyl-, ethyl-, isobutyl-, dimethyl-, isopropyl-, and n-amylamine, pyrrolidine, and piperidine.

FOOTNOTES

1 Research conducted while Dairy Products Laboratory was located in Washington, D.C.






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Copyright © 1974 by the American Dairy Science Association ®.