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Journal of Dairy Science Vol. 57 No. 8 898-899
© 1974 by American Dairy Science Association ®
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Control of Lipase Content in Mucor Rennet Preparations

G. A. Somkuti1

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907

ABSTRACT

The possible removal of lipase activity in concentrated Mucor rennet preparations was investigated. Lipase and protease enzymes were recovered from 5-day old fermentation medium by precipitation with ammonium sulfate. Gel filtration of enzyme mixtures on Sephadex G-75 and Sephadex G-100 resulted in a nearly complete resolution of lipase and protease. The method appeared suitable for preparation of lipase-free fungal rennet.

FOOTNOTES

1 Present address: Lederle Laboratories, Pearl River, N. Y. 10965.






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Copyright © 1974 by the American Dairy Science Association ®.